快速核酸釋放劑（DNA型）-II型    Rapid Nucleic Acid Release Reagent (DNA) - Type II

產品參數Product parameters：

產品特點及優勢Product features and advantages：

產品用途Application：

用於拭子類樣本、植物組織類樣本的預處理和核酸釋放，快速裂解各類常見樣本，無需純化；樣本裂解物可直接作為模板進行擴增反應。

It is used for pre-processing and nucleic acid release of swab samples and plant tissue samples, rapidly cracking all kinds of common samples without purification; The sample lysate can be directly used as a template for amplification reaction.

Tips：其下游結合我司恆溫擴增試劑對核酸進行檢測。

Tips: Its reverse combined with our constant temperature amplification reagent for nucleic acid detection.

操作步驟 Operation procedure and process：

性能對比數據Performance comparison data：

關於bbin宝盈(中国) About us：

bbin宝盈(中国)生物是集恆溫快速核酸擴增底層技術及成品試劑的研發、生產、訂製和銷售於一體的高新技術企業。

Amp- Future Biotech is a high-tech enterprise integrating R&D, production, customization and sales of the underlying technology of isothermal rapid nucleic acid amplification and finished reagents.

團隊歷經十多年的技術沉澱，開發出代表未來生物檢測技術趨勢的多酶恆溫快速擴增技術MIRA（Multienzyme Isothermal Rapid Amplification）。並以MIRA為核心平台技術研發出「超快速核酸釋放技術」、「自測型核酸檢測裝置」、「小型自動化檢測系統」、「雙通道恆溫熒光檢測儀」、「一體化全自動封閉檢測系統」等一系列配套產品，力求打造出快速分子檢測領域的整體解決方案，將MIRA技術的靈敏度高、特異性強、快速簡便、污染風險小、現場作業等特點充分體現出來。

After more than ten years of technical precipitation, the team developed the Multienzyme Isothermal Rapid Amplification technology MIRA (Multienzyme Isothermal Rapid Amplification), which represents the trend of future biological detection technology. With MIRA as the core platform technology, we have developed a series of supporting products such as "ultra-fast nucleic acid release technology", "self-detection nucleic acid detection device", "small automatic detection system", "double-channel isothermal fluorescence detector", "integrated automatic closed detection system", and strive to create an overall solution in the field of rapid molecular detection. The characteristics of MIRA technology, such as high sensitivity, strong specificity, fast and simple, low pollution risk and field operation, are fully demonstrated.

科研客戶涉及各大高校的科学生命學院、醫學院、生物工程學院、農學院、動物學院、水產學院等等，還與疾病預防控制中心、微生物研究所、農業科學院、海關等達成合作。现在已觸達海內外多個地區的科研市場。據不完全統計，已有35+篇SCI文章，還有不少科研轉產項目進行中。

Scientific research customers involve the college of life sciences, medical college, biological engineering college, College of Agriculture, College of Zoology, College of Fisheries, etc., and also cooperate with the Center for Disease Control and Prevention, Microbiology Institute, Academy of Agricultural Sciences, Customs and so on. At present, it has reached the scientific research market in many regions at home and abroad. According to incomplete statistics, there have been 35+ SCI articles, and there are many scientific research conversion projects in progress.

bbin宝盈(中国)將繼續發揮診斷生態的優勢，連結更多海內外科研组织和IVD企業，持續推進，深度融合，促進商機轉化，讓MIRA技術精準快速診斷惠及更多人，守護造福人類生命健康。

In the future, Amp-future will continue to give play to the advantages of diagnostic ecology, link more scientific research institutions and IVD enterprises at home and abroad, continue to promote, deep integration, and promote the transformation of business opportunities, so that MIRA technology accurate and rapid diagnosis can benefit more people, protect and benefit human life and health.

Q：裂解效果差是怎麼回事？

A：樣本須與裂解液充分混合，可用槍頭吹吸或振盪混勻，提高裂解效率。

In order to ensure the cracking effect, the sample must be fully mixed with the cracking liquid, which can be blown or mixed by the gun head or by oscillation to improve the cracking efficiency.

Q：LD-1管結晶凝固了怎麼辦？

A：LD-1 在溫度低於20 ℃環境下易結晶或凝固，使用前可用 30 ℃- 40 ℃水浴或金屬浴儀器進行融解。

LD-1 is easy to crystallize or solidify at temperatures below 20 ℃, and can be melted by 30-40 ℃water bath or metal bath instrument before use.

Q：上清液是否可以作為模板，直接用於擴增反應？

A：渦旋或輕彈振盪混勻後取裂解物上清液作為模板，直接用於擴增反應。

The supernatant of  lysate was used as the template for the amplification reaction after mixing with vortex or flick oscillation.

Q：組織/固體樣本如何處理，取得裂解液呢？

A：組織/固體樣本裂解後的乳濁液或懸濁液可用離心機短暫離心後取上清液作為模板。

The emulsion or suspension of the tissue/solid sample after cracking can be centrifuged for a short time and then the supernatant can be taken as a template.

Q：快速核酸釋放劑（DNA型）-II型是否需要冷鏈運輸？

A：運輸條件：常溫運輸；

Transport conditions: normal temperature transport;

Q：快速核酸釋放劑（DNA型）-II型的保存溫度是什麼？

A：儲存條件：常溫保存；

Storage conditions: room temperature storage; 

Q：快速核酸釋放劑（DNA型）-II型產品有效期多久？

A：產品有效期：12 個月；

Product validity: 12 months; 

Q：快速核酸釋放劑（DNA型）-II型的生產日期位置？

A：生產日期見外包裝；

Production date is on the outer package

Q：快速核酸釋放劑（DNA型）-II型是否需要恆溫加熱？

A：混勻後置於40°C孵育5min，用於上樣檢測；

After mixing, incubate at 40°C for 5min for sample testing;